Human cervix carcinoma established from the epitheloid cervix carcinoma of a 31yearold black woman in 1951. Highly sensitive and specific microrna expression profiling. Expression of folate receptors alpha and beta in normal and. The encoded preproprotein is proteolytically proc essed to generate the 53amino acid epidermal growth factor peptide. The hela cell database includes proteins with important functions and unknown functions, representing today one of the largest twodimensional databases for eukaryotic proteomes and forming the basis for future expressional studies at the protein level. Comprehensive circular rna expression profile in radiationtreated hela cells and analysis of radioresistancerelated circrnas peerj.
For this aim, hela cells were incubated with and aunps and aunrs at various concentrations 5, 10, and 15 gml for 6 hours. The story of henrietta lacks, her family, and the creation of hela cells has been a. Downregulation of pank2 by sirna in hela cells leads to a 12fold. Even though countless other cell lines have been created since 1951, hela was established as the default and remains the most commonly used over 60,000 studies using these cells have been. Here we present a detailed genomic and transcriptomic characterization of a hela cell line. Nov 19, 2015 a few years ago, a book called the immortal life of henrietta lacks, by rebecca skloot was published. Apr 11, 2007 comparison of gene expression profiles of hpl1d and a549 in response to tgf when the gene expression profiles of hpl1d and a549 cell lines in response to tgf. Hela cells by nishi singh is an absorbing glimpse into a strange episode in medical history centered on the life and death of henrietta lacks. M for 48 h were checked using twodimensional electrophoresis 2de. Glycogene expression profiles based on microarray data. The analysis resulted in the identification of about 1200 proteins that were the products of 297 different genes.
The length of hela circrna candidates ranged from 786 rows. During her treatment at johns hopkins hospital, tissue samples of. This study underscores the importance of accounting for the strikingly aberrant characteristics of hela cells when designing and interpreting experiments, and has implications for the use of hela as a model of human biology. Apr 19, 2017 hela cells were the first immortalized human cancer cell line, meaning these cells can continue to divide and grow indefinitely. The fascinating story of the hela cell line and of henrietta lacks from whom these cells originate are described in the book of rebecca skloot cellopub clpub00377. The hela cell line is among the most frequent sources of. We applied proteomics technologies to analyze the proteins of the hela cell. We studied the changes in global gene expression profiles in human cervical cancer hela cells exposed to nonactivated and activated. Aug 07, 2020 hela cell cultures use the immortal cells of henrietta lacks. Materials and methods cell lines and culture hela crl1469, mouse pheomyeloma cell line pc12 crl1721, mouse melanoma cell line b16 crl6475, and hek293 crl1573 cells were purchased from the american type culture collection atcc manassas, va.
Advancements in medical research due to hela cells. Dendrimers and dendriplexes principally affect genes with the molecular functions of nucleic acid binding and transcription activity, metalion binding, enzyme activity, receptor activity, and protein binding. Evaluating the geneexpression profiles of hela cancer cells. The cells have also been found in many cell cultures. The immortal hela genome musings of a mitochondriac. There is a small telocentric chromosome in 98% of the cells. One copy of ml, one copy of m2, fourfive copies of m3, and two copies of m4 as revealed by gbanding patterns.
Chip and reporter assays in hela cells with monoallelic cd177 expression showed that methylation reduced reporter activity, whereas. Microrna expression variability in human cervical tissues. We compared the genomic and transcriptomic profiles of 4 different. Afterward, hela cells were irradiated with 6mv xray at a single dose of 2 gy. Zhou jy, ma wl, liang s, zeng y, shi r, yu hl, xiao ww, zheng wl 2009 analysis of microrna expression profiles during the cell cycle in synchronized hela cells. Was originally considered to be a breast cancer cell line but has since been identified as a melanoma cell line.
The analysis of sequencing data allowed identifying 16,893 circrnas in hela cells. The protein expression profiles of hela cells treated with each ganoderic acid at dose of 15. She was a poor black tobacco farmer whose cells taken without her knowledge in 1951became one of the most important tools in medicine, vital for developing the polio vaccine, cloning, gene mapping, in vitro fertilization, and more. Cell line any a431 a549 af22 asc tert1 bj caco2 efo21 fhdftert166 gamg hacat hap1 hbec3kt hbf tert88 hdlm2 hek 293 hel hela hep g2 htcepi htecsvtert24b hterthme1 htertrpe1 huvec tert2 jurkat k562 lhcnm2 mcf7 nb4 oe19 pc3 reh rh30 rptec tert1 rt4 shsy5y siha skmel30 susa thp1 u2 os u251 mg. Owing to the ease of which they can be effectively synchronized by various methods, hela cells have been used extensively for studies of the cell cycle. Here we describe several protocols for synchronization of hela cells from different phases of the cell cycle. May, 2009 overexpression of tnasp in hela cells significantly affects the expression level of 54 genes 0. Henrietta lacks immortal cells science smithsonian. For this reason, one might anticipate that mirna expression profiles would classify normal human tissues better than the mrna profiles as well. We performed dna and rna sequencing of a hela kyoto cell line and analyzed its mutational portfolio and gene expression profile. Hela cells open books open minds the immortal life of. Cervical cancer is one of the most common cancers in women worldwide. Henriettas cancer cells have been used in unethical research. Egf protein expression summary the human protein atlas.
This is in comparison to primary cells, which continue to age in culture and eventually will stop growing. Northern blot analysis showed that gak is expressed ubiquitously, with the highest level of expression being. Because of this, hela cells have evolved into a useful model to study a huge variety of topics. Human cell line derived from breast adenocarcinoma. However, the mechanism by which nasp exerts its effects in the cell cycle is not understood. By donald zuhn on wednesday, the national institutes of health nih announced that the agency had reached an understanding with the family of ms. Her name was henrietta lacks, but scientists know her as hela. Gene expression profiles of nonsmall cell lung cancer. The book brought the importance of hela cells into the. Henrietta lacks regarding access to genomic data for the hela cell line, which was derived from cervical cancer cells taken from ms. Expression pattern of circrnas during the priming phase of radiationtreated hela cells highthroughput sequencing was used to determine the expression profile of circrnas. Integrated analysis of mrna and mirna expression in hela.
Cultures, supposedly of tissues such as breast cancer or mouse, proved to be hela. English 111 november 15, 2014 english research essay. The correlation between gene expression levels in different samples indicated the biological repetition fig. In hela cells, this was shown to contribute to changes in microrna expression and ultimately to the prolific growth of the cells in culture. Famous hela cells get genetic closeup, and new datasharing. In the present study, a 10 gy xray was applied to hela cells, which were collected 48 h later. Hela cells consist of two cell types, as evidenced by. Jun 12, 2007 even with much less degree of freedom than mrnas, mirna expression profiles reflect the developmental lineage and differentiation state of cells and successfully classified poorly differentiated tumors that could not have definitive diagnosis by histopathology, while the classification based upon the mrna profiles was highly inaccurate.
Gene silencing and a novel monoallelic expression pattern in. Analysis and prediction of pathways in hela cells by. Mitfmir211 axis is a novel autophagy amplifier system during. Expression profiles generated by this method are highly comparable to those obtained with rtpcr r 2 0. Henrietta lacks, a black woman and tobacco farmer in southern virginia, was diagnosed with and died from cervical cancer in 1951. First, to assure the certainty of the gene expression profile of hela cells, we used the rnaseq data set generated by nagaraj et al. The results showed that the addition of aunrs and aunps could enhance the radiosensitivity of hela cells. Twelve proteins including human interleukin17e, eukaryotic translation initiation factor 5a eif5a, peroxiredoxin 2, ubiquilin. E6 expression and sensitizes cells to paclitaxel treatment in hela cells. Jun 15, 2018 comprehensive circular rna expression profile in radiationtreated hela cells and analysis of radioresistancerelated circrnas.
This may be explained by differences in protein expression profiles in hela and ht1080 cells 27 and by the nature of dna employed in these two experiments. We proved that small interfering rnas targeting mapk1 sirna. Utr contains a novel sequence element that binds nxf1. Cancers free fulltext cancer cell lines are useful model. Starting from their stabilization, the hela cells constituted the first example of. Effects of triterpenes from ganoderma lucidum on protein. Mar 18, 20 hela cells were used to study cancer, dissect basic biological processes common to all cells, and develop new technologies to enable further discoveries. Dysregulated microrna profile in hela cell lines induced by lupeol. However, a complete genomewide profile of dysregulated circular rnas.
These findings may improve our understanding of the role of circrnas in radioresistance in hela cells and allow the development of novel therapeutic approaches. However, some of the genes periodically expressed in the hela cell cycle do. Analysis of gene expression profiles in hela cells in response to. Dendrimer activation improved transfection efficiency and induced additional gene expression changes in hela cells. Skloot published a book, the immortal life of henrietta l.
Identification of gene expression profiles in hela cells and. Malignant tumors develop resistance mechanisms and are less sensitive to or do not respond to irradiation. Later diagnosis changed to adenocarcinoma, first aneuploid, continuously cultured human cell line. During the last 10 years, hela has been used to pioneer omics approaches such as microarray based gene expression profiling chaudhry et al. In the field of molecular biology, gene expression profiling is the measurement of the activity the expression of thousands of genes at once, to create a global picture of cellular function. Comprehensive circular rna expression profile in radiation.
Two groups of hela cells were used, and each group contained three samples. Our results provide the first detailed account of genomic variants in the hela genome, yielding insight into their impact on gene expression and cellular function as well as their origins. Characterization of microrna expression profiles in normal. Background nasp nuclear autoantigenic sperm protein is a linker histone chaperone required for normal cell division. Rassmann a, martin u, saluz hp, peter s, munder t, henke a 20 identification of gene expression profiles in hela cells and hepg2 cells infected with coxsackievirus b3. Expression of folate receptors alpha and beta in normal. The analysis of the gene expression profiles of these novel genes. Lacks eventually died of cancer on october 4, 1951. Four typical hela marker chromosomes have been reported in the literature. It was a followup to a readers digest article from some time ago. Materials and methods hela cells and irradiation hela cells were purchased from procell life science and technology co. Overexpression of tnasp in hela cells significantly affects the expression level of 54 genes 0. Open books open minds the immortal life of henrietta lacks by rebecca skloot.
Jun 24, 2011 hela is one of the oldest and most commonly used cell lines in biomedical research. Learn more about hela cells, research, and contamination of cell cultures. Profiling the hela s3 transcriptome using randomly primed cdna and. Knockdown efficiency of af4 was checked by qpcr, using. These profiles can, for example, distinguish between cells that are actively. The altered expression of a number of genes at the mrna level is known to be essential for progression through the cell cycle, however, a comprehensive gene expression profile of human cells. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Activated pi3kaktcox2 pathway induces resistance to. Identification of genes periodically expressed in the human cell. The possible targetrelated proteins of ganoderic acids, i. During the last 10 years, hela has been used to pioneer omics approaches such as microarraybased gene expression profiling chaudhry et al.
Oct 20, 2015 the hela cell line is one of the most popular cell lines in biomedical research, despite its wellknown chromosomal instability. Effects of small interfering rnas targeting mapk1 on gene. Comprehensive circular rna expression profile in radiationtreated hela cells and analysis of radioresistancerelated circrnas circrnas may play a major role in the response to radiation. Gene expression profiling of hela cells in g1 or g2 phases. C optimized highthroughput microrna expression profiling provides novel biomarker. Articles employer profiles find jobs graduate programs. This gene encodes a member of the epidermal growth factor superfamily. High growth rate compared to normal cells, hela cells were shown to grow unusually fast even with their cancerous state taken into consideration. A poor black woman in baltimore, she was diagnosed with cervix cancer and forced to undergo treatment at johns hopkins hospital. Aug 07, 20 the hela cell line was originally derived without permission from a tumor from henrietta lacks, a 31yearold african american woman who died of a particularly aggressive strain of cervical cancer at johns hopkins university in 1951. Aug 01, 20 we compared the gene expression profile of hela with other cell lines and tissues, using data from encode cell lines djebali et al.
Jun 10, 20 there are different gene expression profiles and gene ontologies in the hela and nhek cell lines. Evaluating the geneexpression profiles of hela cancer. Dysregulated microrna profile in hela cell lines induced by. Hela cells were transiently transfected with gfpmitfa vector and incubated with. This method, in conjunction with the 96sample array matrix should prove useful for highthroughput expression profiling of mirnas in large numbers of tissue samples. Effective design and interpretation of molecular genetic studies performed using hela cells require accurate genomic information.
Aug 21, 2017 mirna expression analysis in hela cells upon ncl depletion as previous studies have pointed out to a function of ncl in mirna synthesis 23, 24 we analyzed the effects of ncl depletion in hela. This method, in conjunction with the 96sample array matrix should prove useful for highthroughput expression profiling of mirnas in. However, it should be noted that expression profiles are most often devoid of architectural context and differences may be partly attributable to, or confounded by, the heterogeneity of cell types, and the relative contributions there from, that are potentially present e. Analysis of public rnasequencing data reveals biological. Figure 1a shows a scatter plot summary of upregulated and downregulated genes from cells overexpressing nasp. Analysis of gene expression profiles in hela cells in.
Novel circrnas were identified and annotated using circexplorer zhang et al. The genomic and transcriptomic landscape of a hela cell. Analysis of these variants, and previously unannotated splicing events in the hela s3 cell line, reveals an. Knowledge on protein expression is limited and no comprehensive study on the proteome of this cell type has been reported so far. Hela cells and the ethics behind them from the polio vaccine to heart medicine, hela cells have been a key part of medical research since their discovery in the 1950s. The hela cell database includes proteins with important functions and unknown functions, representing today one of the largest twodimensional databases for eukaryotic proteomes and forming the. The expression profiles of gak and cyclin g during the synchronized hela cell cycle showed that gak expression oscillates slightly, peaking at the g1 phase, although histone h1 kinase activity remained constant throughout the cell cycle. Profiling the hela s3 transcriptome using randomly primed cdna. Pdf gene expression profiling of hela cells in g1 or g2. Changes in nasp expression significantly affect cell growth and development. Dendrimers and dendriplexes principally affect genes with the molecular functions of nucleic acid binding and transcription activity, metalion. Identification of gene expression profiles in hela cells and hepg2 cells infected with coxsackievirus b3. Sep 01, 2020 hela cells, which grew rapidly in cell culture and became the first human cell line, were used by researchers around the world.
Af4 knockdown cells were generated with lentiviral particles, produced by cotransfection of hek293t cells with the shrna plasmid for af4, pcmvdr8. The genomic and transcriptomic landscape of a hela cell line. High variability of genomic instability and gene expression. Jul 01, 2010 the protein expression profiles of hela cells treated with each ganoderic acid at dose of 15. By comparing the normalized gene expression levels of the 16 tissues from the bodymap with those from our hela rnaseq library, we identified 1907 genes of which 805 are proteincoding that were more highly expressed in hela than in any tissue in the bodymap. The comprehensive analysis of radioresistancerelated circrnas may lay a foundation for future studies on the diagnosis and treatment of cervical cancer.
Expression profiling of genes regulated by tgfbeta. Hela cells and the ethics behind them essay 2023 words 9 pages. Transcriptomics analysis of circular rnas differentially. However, 20 years after henrietta lacks death, mounting evidence suggested that hela cells contaminated and overgrew other cell lines. This total expression analysis yielded a set of 19,974 transcripts. In this study, circrna sequencing was used to analyze the circrna expression profile between irradiated hela cells and shamirradiated hela cells and explore the possible involvement of differentiallyexpressed circrnas in radioresistance mechanisms. Protein profile of the hela cell line sciencedirect. One copy of ml, one copy of m2, fourfive copies of m3, and two copies of. Jun 04, 2004 the hela cell database includes proteins with important functions and unknown functions, representing today one of the largest twodimensional databases for eukaryotic proteomes and forming the basis for future expressional studies at the protein level. In 1951, a 30yearold africanamerican woman named henrietta. Hela cells are widely used for all kinds of in vitro studies in biochemistry, biology and medicine. The cell survival ratios were computed by clone formation. The details of the gene expression profiling in efatreated t47d cells have been published. Havoc in biologys mostused human cell line press release.
641 397 113 548 268 984 372 1666 941 383 675 1141 13 1444 1699 1618 285 34 21 126